Journal: Bone Reports

Article Title: Bruton's tyrosine kinase (Btk) inhibitor tirabrutinib suppresses osteoclastic bone resorption

doi: 10.1016/j.bonr.2019.100201

Figure Lengend Snippet: Effects of various inhibitors on mature osteoclast differentiation of human osteoclast precursor cells. Human osteoclast precursor cells were prepared in media containing M-CSF and RANKL. The cells were treated with tirabrutinib, ibrutinib, fostamatinib and tofacitinib at concentrations from 0.1 to 1000 nmol/L for 7 days. Osteoclast differentiation was evaluated by TRAP staining and counting of TRAP+ cells containing at least three nuclei on day 7. (a) Inhibitory effect is expressed as the mean ± standard error ( n = 3 samples per group). ***: p < .001 vs Non-stimulation group. #, ##, ###: p < .05, p < .01, p < .001 vs group with vehicle. (b) Cells after TRAP staining were photographed under a light microscope. All compounds were treated at 100 nmol/L, except for positive control, p38 inhibitor at 10 nmol/L. (c) Cytotoxic effects of tirabrutinib and ibrutinib on human osteoclast precursor cells. After completion of cultivation, RLU was measured. Cytotoxicity is expressed as the mean ± standard error ( n = 3 samples per group).

Article Snippet: Human osteoclast precursor cells (Lonza) were cultured with 33 ng/mL M-CSF and 66 ng/mL RANKL for 7 days according to the manufacturer's protocol (Lonza).

Techniques: Staining, Light Microscopy, Positive Control

Journal: Bone Reports

Article Title: Bruton's tyrosine kinase (Btk) inhibitor tirabrutinib suppresses osteoclastic bone resorption

doi: 10.1016/j.bonr.2019.100201

Figure Lengend Snippet: Btk mediates Osteoclast signaling pathway. (a, b) Primary human osteoclast precursors were stimulated with M-CSF and RANKL for 48 h. Intracellular phosphorylated Btk (pBtk), total Btk protein (Btk), phosphorylated Lyn (pLyn), total Lyn protein (Lyn), phosphorylated Gab2 (pGab2), total Gab2 protein (Gab2), phosphorylated PLCγ2 (pPLCγ2), total PLCγ2 protein (PLCγ2), phosphorylated BLNK, total BLNK and NFATc1 were detected by Western blot analysis. (b) After immunoprecipitation with an anti-Tec antibody, phosphorylated tyrosine (pTyr) and Tec protein (Tec) were detected by Western blot analysis.

Article Snippet: Human osteoclast precursor cells (Lonza) were cultured with 33 ng/mL M-CSF and 66 ng/mL RANKL for 7 days according to the manufacturer's protocol (Lonza).

Techniques: Western Blot, Immunoprecipitation

Journal: Bone Reports

Article Title: Bruton's tyrosine kinase (Btk) inhibitor tirabrutinib suppresses osteoclastic bone resorption

doi: 10.1016/j.bonr.2019.100201

Figure Lengend Snippet: RANKL-RANK signaling pathway via Btk in osteoclast differentiation Tec family kinases are activated by RANK and bind to scaffolding proteins (BLNK or SLP76) activated by ITAM signaling, thereby forming a complex that cooperates to activate PLCγ2. PLCγ2 in turn stimulates calcium signaling, which is required for activation of NFATc1, a transcription factor essential for osteoclast differentiation. Our data suggest that ITAM signaling may be bypassed by RANK-RANKL signaling via Btk/Tec.

Article Snippet: Human osteoclast precursor cells (Lonza) were cultured with 33 ng/mL M-CSF and 66 ng/mL RANKL for 7 days according to the manufacturer's protocol (Lonza).

Techniques: Scaffolding, Activation Assay

Journal: Journal of Indian Society of Periodontology

Article Title: Biomarkers in biological fluids in adults with periodontitis and/or obesity: A meta-analysis

doi: 10.4103/jisp.jisp_512_19

Figure Lengend Snippet: Search Strategy from different databases

Article Snippet: 3. Balli et al ., 2013[ ] , Turkey, University clinic , Prospective; Interventional , 20 , 20 , O+P+: 40.56±4.11 O−P+: 39.67±3.87 , Standards of the international world workshop for classification of periodontal disease and conditions[ ] , GCF Chemerin: ELISA, Hangzhou Eastbiopharm (China);IL-6: ELISA, Boster Biological Technology (USA) , O+P + group showed higher GCF levels of chemerin and IL-6, compared to O−P + group ( P <0.05 and P <0.008 respectively) , Not included in meta-analysis (just Spearman’s rank correlation values of inflammatory markers are shown).

Techniques: Biomarker Discovery, Marker